This book constitutes the refereed proceedings of the Second International Conference on Semantics and Digital Media Technologies, SAMT 2007, held in Genoa, Italy, in December 2007. The 16 revised full papers, 10 revised short papers and 10 poster papers presented together with 3 awarded PhD papers were carefully reviewd and selected from 55 submissions. The conference brings together forums, projects, institutions and individuals investigating the integration of knowledge, semantics and low-level multimedia processing, including new emerging media and application areas. The papers are organized in topical sections on knowledge based content processing, semantic multimedia annotation, domain-restricted generation of semantic metadata from multimodal sources, classification and annotation of multidimensional content, content adaptation, MX: the IEEE standard for interactive music, as well as poster papers and K-space awarded PhD papers.